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Custom synthetic peptides are valuable reagents in biological research, and are widely used to mimic complex protein structures or antigens. Quality of custom peptides, i.e., purity, is the key to success and integrity of your research. Consequently, the main cost for peptide synthesis is incurred on purification and characterization of custom peptides. We have enlisted some of common applications of synthetic peptides and recommended purity for custom synthesis based on our consultations with experts familiar with these biological assays. 

级别

推荐纯度

  应用方向

免疫级别

多肽纯度 >70%

Ø  制备抗原和纯化多克隆抗体

Ø  ELISA 试验(酶联免疫吸附试验)

生化级别

多肽纯度 >85%

Ø  定性的酶底物研究

Ø  磷酸化作用研究

Ø  定性多肽封闭实验

Ø  Coupling to resins for affinity purification

Ø  Coating of tissue culture plates for cell attachment

Ø  用Western blotting方法进行酶的蛋白酶的拮抗实验

Ø  蛋白电泳应用

高纯级别

多肽纯度 >95%

Ø  Standards for quantitative ELISA and RIA protocols定量ELISA和RIA协议标准

Ø  抗原决定簇定位

Ø  定量受体配体相互作用的研究

Ø  质谱分析、定量分析

Ø  定量的磷酸化作用、蛋白水解、蛋白酶的拮抗和竞争研究研究

Ø  NMR 核磁共振研究

Ø  In-vitro bioassays体外生物分析

Ø  In-vivo studies生物体内研究

Ø  Generating standard curves

药用级别

多肽纯度 >98%

Ø  药用多肽、化妆品用多肽

Ø  临床试验

Ø  SAR研究

Ø  X光晶体实验

以上纯度推荐仅做参考,具体指标需根据具体实验确定。