中/英文名称：

ADRA2肾上腺素能受体2抗体/ Anti- ADRA2

别名：

alpha-2A adrenergic receptor; alpha 2a Adrenergic   Receptor; ADRA2A; ADRA2; ADRA2R; ADRAR; ALPHA2AAR; ZNF32; RATRG20; RG20; Adrenergic   alpha 2A receptor; Alpha 2AAR subtype C10; OTTHUMP00000058849; ADA2A_HUMAN;   Alpha-2A adrenergic receptor; Alpha-2 adrenergic receptor subtype C10;   Alpha-2A adrenoreceptor; Alpha-2A adrenoceptor; Alpha-2AAR.

产品编号：

zy10031

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human ADRA2

交叉反应：

Human, Mouse, Rat, Pig, Cow, Rabbit

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 50kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Alpha-2-adrenergic receptors are members of the G   protein-coupled receptor superfamily. They include 3 highly homologous   subtypes: alpha 2A, alpha 2B, and alpha 2C. These receptors have a critical   role in regulating neurotransmitter release from sympathetic nerves and from   adrenergic neurons in the central nervous system. Studies in mouse revealed   that both the alpha 2A and alpha 2C subtypes were required for normal   presynaptic control of transmitter release from sympathetic nerves in the   heart and from central noradrenergic neurons; the alpha 2A subtype inhibited   transmitter release at high stimulation frequencies, whereas the alpha 2C   subtype modulated neurotransmission at lower levels of nerve activity. This   gene encodes alpha 2A subtype and it contains no introns in either its coding   or untranslated sequences. [provided by RefSeq].

其他:

Function:

Alpha-2 adrenergic receptors mediate the   catecholamine-induced inhibition of adenylate cyclase through the action of G   proteins. The rank order of potency for agonists of this receptor is   oxymetazoline > clonidine > epinephrine > norepinephrine >   phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin   = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine   = mianserine > chlorpromazine = spiperone = prazosin > propanolol >   alprenolol = pindolol.

Subcellular   Location:

Cell membrane.

Similarity:

Belongs to the G-protein coupled receptor 1   family. Adrenergic receptor subfamily.

ADRA2A sub-subfamily.

Gene   ID:

150

Tissue/cell: rat brain tissue;   4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for   15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min;   Blocking buffer (normal goat serum) at 37℃ for 20 min;
  Incubation: Anti-ADRA2 Polyclonal Antibody, Unconjugated 1:200, overnight at   4°C, followed by conjugation to the secondary antibody and DAB staining

Protein: line1, rat kidney   lysates, 30ug; line2, rat brain lysates, 30ug;
  Primary: Anti-ADRA2  at 1:200;
  Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000;
  ECL excitated the fluorescence;
  Predicted band size : 50kD
  Observed band size : 50kD