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Anti-AQP3 水通道蛋白-3抗体

Rabbit Anti-AQP3

/英文名称:

水通道蛋白-3抗体/ Anti-AQP3

别名:

AQP 3; AQP3; Aquaporin 3 (GIL blood group);   Aquaporin 3 (Gill blood group); Aquaporin3; Aquaporin-3; GIL; Gill blood   group; AQP3_MOUSE.

产品编号:

zy10002

规格:

0.1ml    0.2ml

抗体来源:

Rabbit

    度:

1mg/ml

缓冲液:

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azideReconstitute with 0.1ml sterile distilled water.

克隆类型:

Polyclonal

 型:

IgG

  原:

KLH conjugated synthetic peptide derived from   mouse AQP3 C-terminus

交叉反应:

Human, Mouse, Rat

状态:

Lyophilized or Liquid

  量:

Predicted molecular weight 32kDa

纯化方法:

affinity purified by Protein A

 

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8, reconstituted.

6 months from date of receipt, -20 to -70, reconstituted.

12 months from date of receipt, -20 to -70as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

 

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

This gene encodes the water channel protein   aquaporin 3. Aquaporins are a family of small integral membrane proteins   related to the major intrinsic protein, also known as aquaporin 0. Aquaporin   3 is localized at the basal lateral membranes of collecting duct cells in the   kidney. In addition to its water channel function, aquaporin 3 has been found   to facilitate the transport of nonionic small solutes such as urea and   glycerol, but to a smaller degree. It has been suggested that water channels   can be functionally heterogeneous and possess water and solute permeation   mechanisms. [provided by RefSeq, Aug 2011]

其他:

Function:

Water channel required to promote glycerol   permeability and water transport across cell membranes. Acts as a glycerol   transporter in skin and plays an important role in regulating SC (stratum   corneum) and epidermal glycerol content. Involved in skin hydration, wound   healing, and tumorigenesis. Provides kidney medullary collecting duct with   high permeability to water, thereby permitting water to move in the direction   of an osmotic gradient. Slightly permeable to urea and may function as a   water and urea exit mechanism in antidiuresis in collecting duct cells. It   may play an important role in gastrointestinal tract water transport and in   glycerol metabolism.

Subcellular   Location:

Membrane; Multi-pass membrane protein.

Tissue   Specificity:

Renal medulla and colon. Predominantly in the inner   medulla. Expressed in basal layer of epidermal keratinocytes.

Similarity:

Belongs to the MIP/aquaporin (TC 1.A.8) family.

Gene   ID: 360

通道蛋白(Channel ProteinAQP 3主要分布于肾脏集合管上皮细胞基侧质膜,除了对水有通透性外、还可转运尿素和甘油等小分子物质,而尿素在尿液浓缩机制中有着重要的作用.有学者认为:AQP-3的阳性率与某些肿瘤分化程度有关(胃癌、结肠癌、乳腺癌、前列腺癌、子宫癌、卵巢癌、甲状腺癌等)

blob.pngAntigen: 0.2ug/100ul

Primary: Antiserum, 1:500,   1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;

Secondary: HRP conjugated   Goat-Anti-Rabbit IgG at 1: 5000;

TMB(C-0024) staining;

Read the data in   MicroplateReader by 450nm.

 

Tissue/cell: rat taste buds;   4% Paraformaldehyde-fixed and paraffin-embedded;

blob.pngAntigen retrieval: citrate   buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous   peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum)   at 37 for 20 min;

Incubation: Anti-AQP-3   Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by   conjugation to the secondary antibody and DAB staining

 

blob.pngTissue/cell: rat tongue tissue; 4%   Paraformaldehyde-fixed and paraffin-embedded;

Antigen retrieval: citrate buffer ( 0.01M, pH   6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen   peroxide for 30min; Blocking buffer (normal goat serum) at 37 for 20   min;

Incubation: Anti-AQP-3 Polyclonal Antibody,   Unconjugated 1:200, overnight at 4°C, followed by conjugation to the   secondary antibody and DAB staining