Rabbit Anti-AQP3 | |
中/英文名称: | 水通道蛋白-3抗体/ Anti-AQP3 |
别名: | AQP 3; AQP3; Aquaporin 3 (GIL blood group); Aquaporin 3 (Gill blood group); Aquaporin3; Aquaporin-3; GIL; Gill blood group; AQP3_MOUSE. |
产品编号: | zy10002 |
规格: | 0.1ml 0.2ml |
抗体来源: | Rabbit |
浓 度: | 1mg/ml |
缓冲液: | 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide;Reconstitute with 0.1ml sterile distilled water. |
克隆类型: | Polyclonal |
亚 型: | IgG |
免 疫 原: | KLH conjugated synthetic peptide derived from mouse AQP3 C-terminus |
交叉反应: | Human, Mouse, Rat |
状态: | Lyophilized or Liquid |
分 子 量: | Predicted molecular weight 32kDa |
纯化方法: | affinity purified by Protein A
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运输与储存 | |
运输: | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
储存: | Use a manual defrost freezer and avoid repeated freezethaw cycles. 1 month from date of receipt, 2 to 8℃, reconstituted. 6 months from date of receipt, -20 to -70℃, reconstituted. 12 months from date of receipt, -20 to -70℃as supplied. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
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产品应用 | |
应用: | WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 |
背景: | This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011] |
其他: | Function: Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism. Subcellular Location: Membrane; Multi-pass membrane protein. Tissue Specificity: Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes. Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family. Gene ID: 360 通道蛋白(Channel Protein)AQP 3主要分布于肾脏集合管上皮细胞基侧质膜,除了对水有通透性外、还可转运尿素和甘油等小分子物质,而尿素在尿液浓缩机制中有着重要的作用.有学者认为:AQP-3的阳性率与某些肿瘤分化程度有关(胃癌、结肠癌、乳腺癌、前列腺癌、子宫癌、卵巢癌、甲状腺癌等) Antigen: 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000; TMB(C-0024) staining; Read the data in MicroplateReader by 450nm.
Tissue/cell: rat taste buds; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-AQP-3 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Tissue/cell: rat tongue tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-AQP-3 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining |