中/英文名称：

载脂蛋白D抗体/ Anti-Apolipoprotein D

别名：

Apolipoprotein D; APOD; APO D; APOD protein;   APOD_HUMAN; Apolipoprotein D; Apo-D; ApoD

产品编号：

zy10003

规格：

0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human APOD

交叉反应：

Human, Mouse, Rat, Dog, Pig, Cow, Rabbit

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 19kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IP=1:20-100    IHC-P=1:100-500  IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Apolipoprotein D (APO D), a glycoprotein involved   in the human plasma lipid transport system. It is a progesterone binding   glycoprotein of 24,000 dalton monomer molecular size, is a constituent of   high density lipoprotein in plasma. The function of apolipoprotein D in the   metabolism of plasma lipoproteins is unclear but the observation that this   protein forms complexes with lecithin: cholesterol acyltransferase has led to   the suggestion that apolipoprotein D may be involved in cholesterol   esterification and transport of substrates and products of the reaction.   Apolipoprotein D is expressed in a range of normal tissues including axillary   apocrine glands, adrenal cortex and corpus luteum. Peripheral nerves,   pituitary, testis, cerebellum and renal tubes are also positive. APO D was   first isolated in large quantity as GCDFP 24, the major protein component of   most human breast cyst fluids. In recent studies, Apo D immunoreactivity has   been shown to be an early indicator of prostate cancer and advanced primary   prostate tumors.

其他:

Function:

APOD occurs in the macromolecular complex with   lecithin-cholesterol acyltransferase. It is probably involved in the   transport and binding of bilin. Appears to be able to transport a variety of   ligands in a number of different contexts.

Subunit:

Homodimer. In plasma, also exists as a   disulfide-linked heterodimer with APOA2.

Subcellular   Location:

Secreted.

Tissue   Specificity:

Expressed in liver, intestine, pancreas, kidney,   placenta, adrenal, spleen, fetal brain tissue and tears.

Post-translational   modifications:

N-glycosylatd. N-glycan heterogeneity at Asn-65:   Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); at Asn-98: Hex5HexNAc4 (minor),   dHex1Hex5HexNAc4 (major), dHex1Hex6HexNAc5 (minor) and dHex1Hex7HexNAc6   (minor).

Similarity:

Belongs to the calycin superfamily. Lipocalin   family.

Gene   ID:347

载脂蛋白D(apolipoprotein D,apoD)是一个糖蛋白,它最初是在人血浆的高密度脂蛋白中被分离出来的。apoD在结构上与其他类型的载脂蛋白存在很大的差异,被归入脂肪促成素家族。apoD可以与胆固醇、黄体酮、胆红素等多种疏水性小分子结合。

apoD在多种脊索动物的各类组织中广泛表达, 在脊椎动物中重要的生理功能。最近的研究表明,apoD可以作为多种癌症及神经系统疾病的早期诊断标记, apoD是一个多配体、多功能的蛋白质.

Tissue/cell: rat brain   tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;

Antigen retrieval: citrate   buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous   peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat   serum) at 37℃ for 20 min;

Incubation: Anti-APOD   Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by   conjugation to the secondary antibody and DAB staining