中/英文名称：

α-1抗胰蛋白酶抗体/ Anti-alpha 1 Antitrypsin

别名：

A1-Antitrypsin; Tryptase; Alpha-1-Antitrypsin;   alpha 1 Antitrypsin; A1A; A1AT; AAT; Alpha 1 antiproteinase; Alpha 1   antitrypsin; Alpha 1 protease inhibitor; alpha-1-AT; alpha1 PI; alpha1   proteinase inhibitor; Clade A (alpha 1 antiproteinase antitrypsin) member 1;   MGC23330; MGC9222; PI; PI; PRO2275; Protease inhibitor 1 (anti elastase);   Serpin A1; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase   antitrypsin) member 1; SerpinA1; A1AT_HUMAN; Alpha-1-antitrypsin; Alpha-1   protease inhibitor; Alpha-1-antiproteinase; Serpin A1.

产品编号：

zy10005

规格：

0.1ml    0.2ml

抗体来源：

Rabbit

浓    度：

1mg/ml

缓冲液：

0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1%   Sodium azide；

Reconstitute with 0.1ml sterile distilled water.

克隆类型：

Polyclonal

亚 型：

IgG

免 疫 原：

KLH conjugated synthetic peptide derived from   human AACT-Alpha1 N-terminus

交叉反应：

Human

状态：

Lyophilized or Liquid

分 子 量：

Predicted molecular weight 45kDa

纯化方法：

affinity purified by Protein A

运输与储存

运输:

The product is shipped at ambient temperature.   Upon receipt, store it immediately at the temperature recommended below.

储存:

Use a manual defrost freezer and avoid repeated   freezethaw cycles.

1 month from date of receipt, 2 to 8℃, reconstituted.

6 months from date of receipt, -20 to -70℃, reconstituted.

12 months from date of receipt, -20 to -70℃as supplied.

When reconstituted in sterile pH 7.4 0.01M PBS or   diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用

应用:

WB=1:100-500    ELISA=1:500-1000    IHC-P=1:100-500    IHC-F=1:100-500  IF=1:100-500
  Not yet tested in other applications.
  Optimal working dilutions must be determined by the end user.

背景:

Alpha 1-Antichymotrypsin, a member of the serine   proteinase inhibitor (serpin) family, inhibits neutrophil proteinase   cathepsin G and mast cell chymases, and protects the lower respiratory tract   from damage by proteolytic enzymes. It contains a reactive centre loop, which   interacts with cognate proteinases, resulting in loop cleavage and a major   conformational change. Recently, alpha 1-antichymotrypsin has been identified   as a major constituent of the neurofibrillary plaques associated with   Alzheimers disease, and in vitro studies have shown that it enhances the rate   of amyloid-fibril formation. These observations and recent genetic evidence   suggest that alpha 1-antichymotrypsin is important in the pathogenesis of   Alzheimers disease.

其他:

Function:

Although its physiological function is unclear,   it can inhibit neutrophil cathepsin G and mast cell chymase, both of which   can convert angiotensin-1 to the active angiotensin-2.

Subunit:

Interacts with DNAJC1.

Subcellular   Location:

Secreted.

Tissue   Specificity:

Plasma. Synthesized in the liver. Like the   related alpha-1-antitrypsin, its concentration increases in the acute phase   of inflammation or infection. Found in the amyloid plaques from the   hippocampus of Alzheimer disease brains.

Similarity:

Belongs to the serpin family.

Gene   ID: 12

AACT是由神经胶质细胞分泌并参与淀粉样纤维生成和沉积的一种色氨酸蛋白酶抑制剂,被认为是Aβ肽的假性底物，与Aβ肽具有很高的亲合性，可促进Aβ肽的纤维化以生成淀粉样沉积。

ApoE作为AD的另一种病理伴随性物质，也可识别并与Aβ肽相结合，促进Aβ肽沉积生成淀粉样老年斑块。由于AACT和ApoE在AD发病中具有共同的作用途径，因此探讨其相互作用可能是AD治疗中一个新的途径。

AACT是一种丝氨酸蛋白酶抑制剂，可以中和糜蛋白酶等酶活性的蛋白酶，存在于大多数的组织细胞、巨噬细胞以及多种胃肠道和肺部肿瘤中，但多形核白细胞中无此物质，它可以作为组织细胞瘤和良性/恶性纤维组织细胞瘤的标志。此抗体与人的AACT反应，主要用于恶性纤维组织细胞瘤等恶性肿瘤的诊断,也可用于胃癌﹑肺癌、肾癌等肿瘤的研究。